Part:BBa_K207001:Design

PhyB-DBD Fusion for Yeast-Two-Hybrid

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 490
  Illegal BamHI site found at 572
  Illegal XhoI site found at 523
  Illegal XhoI site found at 542
  Illegal XhoI site found at 2081
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2000
  Illegal SapI site found at 739
  

Design Notes

This is not the complete PhyB sequence, it is just the N terminus (the portion needed to function while minimizing background), residues 1-621.

Source

Part cloned from cDNA from Arabidopsis thaliana. The DBD and NS are from pGBKT7.

The part is cloned into registry plasmid pSB12A, Amp resistant.